Association of the Single-Nucleotide Polymorphism and Haplotype of the Complement Receptor 1 Gene with Malaria.
10.3349/ymj.2015.56.2.332
- Author:
Yan LAN
1
;
Chuan Dong WEI
;
Wen Cheng CHEN
;
Jun Li WANG
;
Chun Fang WANG
;
Guo Gang PAN
;
Ye Sheng WEI
;
Le Gen NONG
Author Information
1. Department of Dermatology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, P. R. China.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Erythrocyte complement receptor type 1;
gene;
polymorphism;
haplotype;
malaria
- MeSH:
Adult;
Alleles;
Asian Continental Ancestry Group;
Case-Control Studies;
China;
Erythrocytes/parasitology;
Female;
Genetic Predisposition to Disease;
Genotype;
*Haplotypes;
Humans;
Malaria/ethnology/*genetics;
Male;
Middle Aged;
Polymorphism, Single Nucleotide/*genetics;
Promoter Regions, Genetic/*genetics;
Receptors, Complement/blood/*genetics;
Taq Polymerase
- From:Yonsei Medical Journal
2015;56(2):332-339
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Although the polymorphisms of erythrocyte complement receptor type 1 (CR1) in patients with malaria have been extensively studied, a question of whether the polymorphisms of CR1 are associated with severe malaria remains controversial. Furthermore, no study has examined the association of CR1 polymorphisms with malaria in Chinese population. Therefore, we investigated the relationship of CR1 gene polymorphism and malaria in Chinese population. MATERIALS AND METHODS: We analyzed polymorphisms of CR1 gene rs2274567 G/A, rs4844600 G/A, and rs2296160 C/T in 509 patients with malaria and 503 controls, using the Taqman genotyping assay and PCR-direct sequencing. RESULTS: There were no significant differences in the genotype, allele and haplotype frequencies of CR1 gene rs2274567 G/A, rs4844600 G/A, and rs2296160 C/T polymorphisms between patients with malaria and controls. Furthermore, there was no association of polymorphisms in the CR1 gene with the severity of malaria in Chinese population. CONCLUSION: These findings suggest that CR1 gene rs2274567 G/A, rs4844600 G/A, and rs2296160 C/T polymorphisms may not be involved in susceptibility to malaria in Chinese population.
