3-Deoxysappanchalcone Promotes Proliferation of Human Hair Follicle Dermal Papilla Cells and Hair Growth in C57BL/6 Mice by Modulating WNT/β-Catenin and STAT Signaling.
10.4062/biomolther.2016.183
- Author:
Young Eun KIM
1
;
Hyung Chul CHOI
;
In Chul LEE
;
Dong Yeon YUK
;
Hyosung LEE
;
Bu Young CHOI
Author Information
1. Cosmecutical R&D Center, HP&C, Seowon University, Cheongju 28674, Republic of Korea.
- Publication Type:Original Article
- Keywords:
3-deoxysappanchalcone;
Human hair follicle dermal papilla cells;
WNT/β-catenin;
STAT3;
STAT6;
C57BL/6 mice
- MeSH:
Animals;
Blotting, Western;
Fibroblast Growth Factors;
Hair Follicle*;
Hair*;
Humans*;
Interleukin-6;
Luciferases;
Mice*;
Phosphorylation;
Phosphotransferases;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
T-Lymphocytes;
Transcriptional Activation;
Transducers;
Vascular Endothelial Growth Factor A
- From:Biomolecules & Therapeutics
2016;24(6):572-580
- CountryRepublic of Korea
- Language:English
-
Abstract:
3-Deoxysappanchalcone (3-DSC) has been reported to possess anti-allergic, antiviral, anti-inflammatory and antioxidant activities. In the present study, we investigated the effects of 3-DSC on the proliferation of human hair follicle dermal papilla cells (HDPCs) and mouse hair growth in vivo. A real-time cell analyzer system, luciferase assay, Western blot and real-time polymerase chain reaction (PCR) were employed to measure the biochemical changes occurring in HDPCs in response to 3-DSC treatment. The effect of 3-DSC on hair growth in C57BL/6 mice was also examined. 3-DSC promoted the proliferation of HDPCs, similar to Tofacitinib, an inhibitor of janus-activated kinase (JAK). 3-DSC promoted phosphorylation of β-catenin and transcriptional activation of the T-cell factor. In addition, 3-DSC potentiated interleukin-6 (IL-6)-induced phosphorylation and subsequent transactivation of signal transducer and activator of transcription-3 (STAT3), thereby increasing the expression of cyclin-dependent kinase-4 (Cdk4), fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF). On the contrary, 3-DSC attenuated STAT6 mRNA expression and IL4-induced STAT6 phosphorylation in HDPCs. Finally, we observed that topical application of 3-DSC promoted the anagen phase of hair growth in C57BL/6 mice. 3-DSC stimulates hair growth possibly by inducing proliferation of follicular dermal papilla cells via modulation of WNT/β-catenin and STAT signaling.
