DW2007 Ameliorates Colitis and Rheumatoid Arthritis in Mice by Correcting Th17/Treg Imbalance and Inhibiting NF-κB Activation.
10.4062/biomolther.2016.018
- Author:
Su Min LIM
1
;
Sang Yun LEE
;
Jin Ju JEONG
;
Hyun Sik CHOI
;
Hwan Bong CHANG
;
Dong Hyun KIM
Author Information
1. Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea. dhkim@khu.ac.kr
- Publication Type:Original Article
- Keywords:
DW2007;
Colitis;
Arthritis;
Th17 Cell;
Treg cell
- MeSH:
Adaptive Immunity;
Anemarrhena;
Animals;
Arthritis;
Arthritis, Experimental;
Arthritis, Rheumatoid*;
Cell Differentiation;
Colitis*;
Colon;
Coptis;
Dacarbazine;
Immunity, Innate;
In Vitro Techniques;
Macrophage Activation;
Mice*;
Mucous Membrane;
Peroxidase;
Rhizome;
Spleen;
T-Lymphocytes, Regulatory;
Th17 Cells
- From:Biomolecules & Therapeutics
2016;24(6):638-649
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the previous study, the rhizome mixture of Anemarrhena asphodeloides and Coptis chinensis (DW2007), improved TNBS-, oxazolone-, or DSS-induced colitis in mice by regulating macrophage activation. Therefore, to understand the effect of DW2007 on the T cell differentiation involved in the adaptive immunity, we measured its effect on both Th17 and Treg cell differentiation in splenocytes, in the lamina propria of mice with DSS-induced colitis (DIC), and in the spleens of mice with collagen-induced arthritis (CIA). Results showed that DW2007 potently inhibited the differentiation of splenocytes into Th17 cells, but increased Treg cell differentiation in vitro. In the colon of wild type and TLR4−/− mice with DIC, DW2007 potently suppressed DSS-induced colon shortening and myeloperoxidase activity. DW2007 also suppressed collagen-induced paw thickening, clinical index, and myeloperoxidase activity in CIA mice. Overall, DW2007 potently suppressed Th17 cell differentiation in mice with CIA and DIC, but increased Treg cell differentiation. Moreover, DW2007 strongly inhibited the expression of TNF-α and IL-1β, as well as the activation of NF-κB. Based on these findings, DW2007 may ameliorate inflammatory diseases by regulating the innate immunity via the inhibition of macrophage activation and the adaptive immunity via the correction of disturbed Th17/Treg cells.
