Proliferation of Corneal Endothelial Cells by Delivery of SV40 Large T Antigen.
- Author:
Kyung Sun CHO
1
;
In Kyung SONG
;
Timothy P FLEMING
;
Choun Ki JOO
Author Information
1. Department of Ophthalmology and Visual Science, Kangnam St. Mary's Hospital.
- Publication Type:Original Article
- Keywords:
BrdU;
Cell proliferation;
Ki-67;
Protein delivery;
SV40 large T antigen
- MeSH:
Antigens, Viral, Tumor*;
Bromodeoxyuridine;
Cell Proliferation;
DNA;
Endothelial Cells*;
Immunohistochemistry
- From:Journal of the Korean Ophthalmological Society
2001;42(9):1331-1335
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To determine whether the delivery of the SV40 large T-antigen is a feasible method for transiently inducing proliferation of corneal endothelial cells, we delivered liposome-protein complex into bovine corneal endothelial cells(BCEC). METHOD: SV40 large T-antigen protein was introduced into BCEC and positive cells were identified by immunohistochemistry. Quiescent BCECs were double-labeled using BrdU as a measure of de novo DNA synthesis and the Ki-67 was detected by standard immunohistochemical methods. RESULT: The treatment of quiescent BCECs with large T antigen caused an increase in BrdU incorporation and Ki-67 expression. It was tested by time-course study. CONCLUSION: This finding suggests that liposome-mediated delivery of transforming proteins could be a method to transiently induce corneal endothelial cell proliferation.
