Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis.
- Author:
Sun Ouck KIM
1
;
Seung Hee SONG
;
Seung Chul LEE
;
Kyung A CHO
;
Ho Song YU
;
In Sang HWANG
;
Eu Chang HWANG
;
Dongdeuk KWON
Author Information
1. Department of Urology, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea. seinsena@hanmail.net
- Publication Type:Original Article
- Keywords:
Caveolin 1;
Cystitis;
Rats
- MeSH:
Animals;
Blotting, Western;
Capillaries;
Caveolin 1;
Contracts;
Cyclophosphamide;
Cystitis;
Female;
Humans;
Injections, Intraperitoneal;
Rats;
Rats, Sprague-Dawley;
Urinary Bladder;
Urodynamics;
Venules
- From:International Neurourology Journal
2012;16(4):169-174
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The purposes of this study were to investigate the effect of cyclophosphamide (CYP)-induced inflammatory cystitis on caveolin 1 in rat urinary bladder and to determine the role of these molecules in the bladder dysfunction that occurs in inflammatory change in rat urinary bladder. METHODS: Female Sprague-Dawley rats were divided into control (n=30) and experimental (n=30) groups. Cystitis in experimental group was induced by intraperitoneal injection of CYP (200 mg/kg). The control group underwent an intraperitoneal saline injection. After 3 days, urodynamic studies were done to measure the contraction interval and contraction pressure. The expression and cellular localization of caveolin 1 were determined by Western blot and immunofluorescent study in rat urinary bladder. RESULTS: In cystometrograms, the contraction interval (minute) was significantly increased in the CYP-induced cystitis rats (15.8+/-1.5) than in the control group (6.3+/-0.5) (P<0.05). Conversely, the average contraction pressure (mmHg) was significantly higher in the CYP-induced cystitis rats (15.6+/-1.7) than in the control group (11.3+/-0.5) (P<0.05). Caveolin 1 was expressed in the capillaries, arteriols and venules. The protein expression of caveolin 1 was significantly decreased in the CYP-induced cystitis rats (P<0.05). CONCLUSIONS: Inflammatory change of urinary bladder maybe causes a significant change in the expression of caveolin 1. These findings suggest that caveolin 1 might have a functional role in the bladder dysfunction related with cystitis in rat urinary bladder.
