Association of IL-10 genotypes with cardiovascular risk factors in patients with hemodialysis.
- Author:
Gang Jee KO
1
;
Jeong Yup KIM
;
Myung Kyu KIM
;
Soon Yong SUH
;
Hye Min CHOI
;
Young Youl HYUN
;
Chang Su BOO
;
Jee Eun LEE
;
Su Ah SUNG
;
Sang Kyung JO
;
Won Yong CHO
;
Hyeong Gyu KIM
Author Information
1. Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea. wonyong@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Interleukin-10;
Polymorphism;
Inflammation;
Cardiovascular disease
- MeSH:
Alleles;
Atherosclerosis;
Cardiovascular Diseases;
Creatinine;
Echocardiography;
Enzyme-Linked Immunosorbent Assay;
Genotype*;
Hand;
Humans;
Hypertrophy, Left Ventricular;
Inflammation;
Interleukin-10*;
Kidney Failure, Chronic;
Malnutrition;
Mortality, Premature;
Polymerase Chain Reaction;
Renal Dialysis*;
Risk Factors*;
Uremia;
Uric Acid
- From:Korean Journal of Medicine
2005;68(5):528-536
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Chronic systemic inflammation in ESRD patients due to uremia and hemodialysis procedure itself comes into notice as a main factor for premature mortality secondary to rapid progressing atherosclerosis. Various pro-inflammatory cytokine, known to mediate these reaction of malnutrition, inflammation and atherosclerosis, are regulated by anti-inflammatory cytokine, such as IL-10. Quantitative production of IL-10 shows interindividual variability determined genetically by polymorphisms of promotor gene. The aim of this study was to measure the degree of IL-10 synthesis in ESRD patients treated with hemodialysis and evaluate the association with genotypes and cardiovascular risk factors. METHODS: The IL-10 genotypes for polymorphic bases at position at -1082 was determined in 66 chronic hemodialysis patients and 98 healthy subjects using highly specific PCR and the lipopolysaccharide (LPS)-stimulated IL-10 (sIL-10) release from whole blood were measured by ELISA. RESULTS: The distribution of the IL-10 genotypes in hemodialysis patients were similar to the general population, but the proportion of A allele in hemodialysis group was significantly higher (72.3% vs 59.8%, p=0.05). sIL-10 concentration were lower in hemodialysis patients compared with normal control (21.1 pg/mg vs 36.1 pg/mg, p=0.001) and both groups showed same relationship of sIL-10 with genotypes, that AA type was low producer. In multiple regression analysis, sIL-10 of normal group correlated negatively with age, creatinine, uric acid and existence LVH, and positively with albumin, hemoglobin. On the other hand, lower albumin, lower ejection fraction on echocardiography and existence of left ventricular hypertrophy were associated with higher sIL-10 in hemodialysis group. CONCLUSION: Polymorphisms by IL-10 genotypes were associated with production of IL-10 by endotoxin stimulation, and sIL-10 was lower in hemodialysis patients than in normal control. According to relation of sIL-10 with cardiovascular risk factors such as existence LVH, ejection fraction and malnutrition, it could be suggested that sIL-10 is useful marker in evaluating the risk of cardiovascular events.
