Human salivary gland stem cells ameliorate hyposalivation of radiation-damaged rat salivary glands.
- Author:
Jaemin JEONG
1
;
Hyunjung BAEK
;
Yoon Ju KIM
;
Youngwook CHOI
;
Heekyung LEE
;
Eunju LEE
;
Eun Sook KIM
;
Jeong Hun HAH
;
Tack Kyun KWON
;
Ik Joon CHOI
;
Heechung KWON
Author Information
1. Division of Radiation Oncology, Korea Institute of Radiological and Medical Sciences, Seoul, Korea. hck@kirams.re.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
amylase;
hyposalivation;
radiation-induced damage;
salivary gland;
stem cells
- MeSH:
Amylases/genetics/metabolism;
Animals;
Antigens, CD/genetics/metabolism;
Apoptosis;
Cell Differentiation;
Humans;
Male;
Mesenchymal Stromal Cells/*cytology/metabolism;
Radiation Injuries, Experimental;
Rats;
Rats, Wistar;
*Regeneration;
Salivary Glands/cytology/injuries/physiology/*surgery;
*Salivation;
*Stem Cell Transplantation
- From:Experimental & Molecular Medicine
2013;45(11):e58-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Salivary function in mammals may be defective for various reasons, such as aging, Sjogren's syndrome or radiation therapy in head and neck cancer patients. Recently, tissue-specific stem cell therapy has attracted public attention as a next-generation therapeutic reagent. In the present study, we isolated tissue-specific stem cells from the human submandibular salivary gland (hSGSCs). To efficiently isolate and amplify hSGSCs in large amounts, we developed a culture system (lasting 4-5 weeks) without any selection. After five passages, we obtained adherent cells that expressed mesenchymal stem cell surface antigen markers, such as CD44, CD49f, CD90 and CD105, but not the hematopoietic stem cell markers, CD34 and CD45, and that were able to undergo adipogenic, osteogenic and chondrogenic differentiation. In addition, hSGSCs were differentiated into amylase-expressing cells by using a two-step differentiation method. Transplantation of hSGSCs to radiation-damaged rat salivary glands rescued hyposalivation and body weight loss, restored acinar and duct cell structure, and decreased the amount of apoptotic cells. These data suggest that the isolated hSGSCs, which may have characteristics of mesenchymal-like stem cells, could be used as a cell therapy agent for the damaged salivary gland.
