Effects of Chitosan on the Differentiation of MDPC-23 Cells.
- Author:
Ju Hyun PARK
1
;
Do Kyung KIM
;
Jong Tae PARK
;
Su Young KIM
;
Sun Kyoung YU
;
Kwang Hee CHO
;
Heung Joong KIM
Author Information
1. Oral Biology Research Institute, Chosun University School of Dentistry, Gwangju, Korea. hjbkim@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
chitosan;
odontoblast;
differentiation
- MeSH:
Animals;
Chitosan;
Dental Papilla;
Dentin;
Mice;
Odontoblasts;
Regeneration;
RNA, Messenger
- From:International Journal of Oral Biology
2010;35(3):91-97
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effects of chitosan upon the experimentally induced differentiation of MDPC-23 cells, derived from mouse dental papilla cells, were investigated by RT-PCR, observations of cell morphology and Alizaline red-S staining. Chitosan was found to significantly increase and accelerate the expression of ALP mRNA but decrease the ColI transcript levels, as compared with the control, in a time-dependent manner during the differentiation of MDPC-23 cells. Chitosan also significantly downregulated ON mRNA expression and accelerated mineralization in differentiating MDPC-23 cells. These results suggest that chitosan facilitates odontoblast differentiation and mineralization and may have potential clinical applications as a dentin regeneration material.
