Characterization of Lactobacillus reuteri BCLR-42 and Lactobacillus plantarum BCLP-51 as novel dog probiotics with innate immune enhancing properties.
10.14405/kjvr.2016.56.2.75
- Author:
Eun Jin KIM
1
;
Yeong Im KANG
;
Tae Il BANG
;
Myoung Han LEE
;
Sang Won LEE
;
In Soo CHOI
;
Chang Seon SONG
;
Joong Bok LEE
;
Seung Yong PARK
Author Information
1. Laboratory of Veterinary Immunology, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea. paseyo@konkuk.ac.kr
- Publication Type:Original Article
- Keywords:
dog;
feces;
in vitro innate immune activity;
lactic acid bacteria;
probiotics
- MeSH:
Alkaline Phosphatase;
Animals;
Anti-Bacterial Agents;
Bacteria;
beta-Galactosidase;
Bile;
Dogs*;
Feces;
Gentamicins;
Granulocytes;
Hydrogen-Ion Concentration;
Lactobacillus plantarum*;
Lactobacillus reuteri*;
Lactobacillus*;
Metronidazole;
Phagocytosis;
Probiotics*;
Respiratory Burst;
Vancomycin
- From:Korean Journal of Veterinary Research
2016;56(2):75-84
- CountryRepublic of Korea
- Language:English
-
Abstract:
Probiotics that are able to provide beneficial effects on animal health have become important ingredients of dog foods. This study was conducted to characterize the probiotic potentials of two strains, Lactobacillus reuteri BCLR-42 and Lactobacillus plantarum BCLP-51, that were derived from feces of healthy dogs and evaluated based on tolerance to low pH and bile acid, antimicrobial activities, enzyme profiles, sensitivity to antibiotics, and innate immune enhancing potentials. Both strains showed survival of more than 90% at pH 3 and 0.2% bile acid and exhibited broad antimicrobial activities against indicator bacteria. Moreover, both strains showed high sensitivity to antibiotics, except vancomycin, metronidazole, and gentamicin. The alkaline phosphatase was negligible (score 0), whereas they showed strong beta galactosidase activity (score range 5 or 3, respectively). The phagocytosis and oxidative burst activities of canine granulocytes were significantly enhanced in response to both strains. These results show that both strains have the capability to act as probiotics and the potential for application as ingredients in dog foods.
