Comparison of Six Clinical Chemistry Test Results According to the Treatment of EDTA Anticoagulant in Body Fluid Specimens.
- Author:
Donghyun LEE
1
;
Jae Yun JANG
;
Kwang Ro YOON
;
Hanjun KIM
;
Young UH
;
Juwon KIM
;
Kap Jun YOON
Author Information
1. Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea. u931018@yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Body fluids;
Clinical chemistry tests;
Anticoagulants
- MeSH:
Adenosine Deaminase;
Anticoagulants;
Body Fluids;
Bronchoalveolar Lavage;
Bronchoalveolar Lavage Fluid;
Cell Count;
Chemistry, Clinical;
Clinical Chemistry Tests;
Edetic Acid;
Glucose;
L-Lactate Dehydrogenase
- From:Journal of Laboratory Medicine and Quality Assurance
2012;34(2):87-92
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Specimen requirements such as type of anticoagulant and number of tube for body fluid analysis vary with specimen type and requested laboratory tests. We compared the results of six clinical chemistry tests between EDTA anticoagulated and anticoagulant-free body fluids. METHODS: A total of 191 body fluids (45 pleural, 28 bronchoalveolar lavage, 35 peritoneal, 45 peritosol, and 38 synovial fluids) were aliquoted into EDTA tubes and anticoagulant-free tubes, and were simultaneously tested for total protein, albumin, glucose, lactate dehydrogenase, adenosine deaminase, and amylase. RESULTS: The coefficient of determination (R2) for all six clinical chemistry test results between EDTA anticoagulated and anticoagulant-free body fluids are more than 0.95 with the exception of glucose in bronchoalveolar lavage fluid (R2= 0.78). CONCLUSIONS: EDTA anticoagulated specimen could be used for testing routinely requested clinical chemistry tests in body fluid analysis, that only one tube of specimen is necessary to perform cell count, differential count, and clinical chemistry tests.