Comparison of Rapid Antigen Test and Real-Time Reverse Transcription PCR for the Detection of Influenza B Virus.
- Author:
Min Jeong PARK
1
;
Han Sung KIM
;
Young Kyung LEE
;
Hee Jung KANG
;
Jae Seok KIM
;
Wonkeun SONG
;
Kyu Man LEE
Author Information
1. Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, Korea. kimhan@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
Influenza B virus;
Rapid antigen test;
Real-time PCR
- MeSH:
Humans;
Influenza A virus;
Influenza B virus;
Influenza, Human;
Polymerase Chain Reaction;
Real-Time Polymerase Chain Reaction;
Reverse Transcription;
Sensitivity and Specificity
- From:Journal of Laboratory Medicine and Quality Assurance
2012;34(2):93-97
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Rapid antigen test and real-time reverse transcription PCR (rRT-PCR) are widely used for the detection of influenza A virus. In this study, we evaluated and compared the effectiveness of a rapid antigen test, currently used for detecting influenza B virus, with the effectiveness of using rRT-PCR for the same purpose. METHODS: Samples obtained from 92 patients during an outbreak of influenza B were assessed using the rapid antigen test (SD BIOLINE Influenza Ag; SD, Korea) and rRT-PCR (Anyplex FluA/B Real-time Detection; Seegene, Korea). RESULTS: The sensitivity and specificity of the rapid antigen test were 69% and 100%, respectively, in detecting influenza B when compared to rRT-PCR. Twenty-nine patients (31.5%) were positive for both rapid antigen test and rRT-PCR, while 50 (54.3%) were negative for both rapid antigen test and rRT-PCR. The overall concordance rate between rapid antigen test and rRT-PCR was 85.9%. Thirteen patients (14.1%) were positive only for rRT-PCR. CONCLUSIONS: The rapid antigen test showed high specificity and good correlation with rRT-PCR and is likely to be as useful in the detection of influenza B viruses.
