The relationship between interleukin-6 gene G(-634)C polymorphism and change in bone mineral density after hormone replacement therapy in postmenopausal Korean women.
- Author:
Dong Yun LEE
1
;
Jung Gu KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Postmenopausal women;
IL-6 gene;
G(-634)C polymorphism;
BMD;
HRT
- MeSH:
Absorptiometry, Photon;
Blood Cells;
Bone Density*;
Enzyme-Linked Immunosorbent Assay;
Female;
Femur;
Genotype;
Hormone Replacement Therapy*;
Humans;
Immunoassay;
Interleukin-6*;
Osteocalcin;
Receptors, Interleukin-6;
Spine
- From:Korean Journal of Obstetrics and Gynecology
2005;48(9):2148-2156
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the relationship among the G(-634)C polymorphism in interleukin-6 (IL-6) gene, change in production of IL-6 by whole blood cells (WBCs), and bone mineral density (BMD) after hormone replacement therapy (HRT) in postmenopausal Korean women. METHODS: The IL-6 G(-634)C polymorphism was analyzed by restriction fragment length polymorphsim (REFLP) in 194 postmenopausal Korean women receiving sequential HRT for 1 year. IL-6 and soluble IL-6 receptor (sIL-6r) produced by WBCs cultured with lipopolysaccharide for 2 days, and serum CrossLaps (CTX) and osteocalcin were measured using enzyme-linked immunosorbent assay (ELISA) and immunoassay respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry. RESULTS: The annual percent changes of BMD were not associated with IL-6 genotypes, and the distribution of IL-6 genotypes were not different between HRT-responders and HRT-nonresponders (women who lose more than 3% of bone mass per year). After HRT of 6 months, an increase in IL-6 production by WBCs, without change in sIL-6r production was noted. There were no differences in the 6 month changes of bone turnover markers and IL-6 production by WBCs after HRT across IL-6 genotypes. Change in IL-6 production by WBCs after HRT of 6 month did not correlated with changes of bone turnover markers, but correlated negatively with annual change of BMD at trochanter after HRT. CONCLUSION: The IL-6 G(-634)C polymorphism did not associate with change in BMD after HRT in postmenopausal Korean women, and did not affect change in the production of IL-6 and sIL-6r by WBCs.
