Detection of C-erbB-2 Internal Domain in Bladder Tumor.
- Author:
Wun Jae KIM
1
;
Hyun Moo LEE
;
Hyung Geun SONG
Author Information
1. Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.
- Publication Type:Original Article
- Keywords:
Bladder tumor;
BBN;
C-erbB-2 internal domain
- MeSH:
Animals;
Carcinoma, Squamous Cell;
Carcinoma, Transitional Cell;
Cell Membrane;
Humans;
Immune Sera;
Oncogene Proteins;
Paraffin;
Rats;
Receptor, erbB-2;
Retrospective Studies;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
1994;35(10):1041-1046
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
c-erbB-2 overexpression appears to play a role in determining the malignant potential of some human cancers. Using a specific monoclonal antibody to c-erbB-2 internal domain, 25 transitional cell carcinoma and I squamous cell carcinoma specimens of the human bladder, and 30 cases of BBN induced rat bladder tumors were analyzed immunohistochemically to detect the expression of this putative transmembrane receptor. c-erbB-2 internal domain was not expressed in rat urinary bladder carcinomas induced by BBN, but in 32% (8/25) of human transitional cell carcinomas. Although c-erbB-2 was expressed in human bladder tumor, there was no correlation between expression of this oncoprotein and characteristics of bladder tumor. Positivity for c-erbB-2 oncoprotein was easily identifiable as an intense red staining localized predominantly at the cell membrane, supporting the view that c-erbB-2 protein is a transmembrane molecule. In most of the positive tumors, the distribution of staining is heterogenous. This suggests that small populations of tumor cells that have elevated levels of c-erbB-2 oncoprotein can be detected by staining. The availability of antisera that detect many oncoproteins in paraffin embedded tissue makes it feasible to design retrospective and prospective studies on selected groups of patients.
