Evaluation of ChromID MRSA for the Detection of Methicillin-resistant Staphylococcus aureus.
10.5145/KJCM.2009.12.4.169
- Author:
Min Jung KIM
1
;
Dae Hyuk KANG
;
Jae Im PARK
;
Tae Yeal CHOI
Author Information
1. Department of Laboratory Medicine, Hanyang University Hospital, Seoul, Korea. tychoi@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Methicillin-resistant Staphylococcus aureus (MRSA);
Detection;
Performance;
Culture medium
- MeSH:
Agar;
Humans;
Infant, Newborn;
Infant, Premature;
Infection Control;
Intensive Care Units;
Mannitol;
Mass Screening;
Methicillin Resistance;
Methicillin-Resistant Staphylococcus aureus;
Oxacillin;
Sensitivity and Specificity
- From:Korean Journal of Clinical Microbiology
2009;12(4):169-173
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant pathogen in both nosocomial and community settings, and screening for a carrier is an important infection control practice in many hospitals. We evaluated the sensitivity and specificity of the ChromID MRSA assay (bioM?rieux, Marcy I'Etoile, France). METHODS: A total of 190 clinical samples were collected from the anterior nares of premature infants in a newborn intensive care unit (N-ICU). Equal volumes (100microliter) of the samples were inoculated on mannitol salt agar with oxacillin 6 mg/L (MSAO) and ChromID MRSA after emulsifying the screening swab in brain-heart Infusion broth with oxacillin 6 mg/L (BE). The specimens in BE were subcultured on ChromID MRSA after an overnight incubation. RESULTS: Twenty-one of 190 samples (11%) was positive for MRSA by BE. After a 24 h incubation, the sensitivity/specificity of MSAO was 52%/98% and that of ChromID MRSA was 62%/100%, and at 48 h, the sensitivity/specificity of MSAO was 62%/92% and that of ChromID MRSA was 81%/99%. CONCLUSION: ChromID MRSA is a useful selective medium for the rapid isolation and identification of MRSA.
