Colonization Rate, Serotypes, and Distributions of Macrolide-Lincosamide-Streptogramin(B) Resistant Types of Group B Streptococci in Pregnant Women.
10.5145/KJCM.2009.12.4.174
- Author:
Young UH
1
;
Seong Jin CHOI
;
In Ho JANG
;
Kwan Soo LEE
;
Hyun Mi CHO
;
Ohgun KWON
;
Kap Jun YOON
Author Information
1. Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea. u931018@yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Group B streptococci;
Erythromycin;
Clindamycin;
erm(B);
Serotype
- MeSH:
Agar;
Clindamycin;
Colon;
Culture Media;
Erythromycin;
Female;
Humans;
Incidence;
Polymerase Chain Reaction;
Pregnant Women;
Prevalence;
Serotyping;
Streptococcal Infections;
Vagina
- From:Korean Journal of Clinical Microbiology
2009;12(4):174-179
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The prevalence of neonatal group B streptococcal infection depends mainly on the colonization rate of pregnant women by group B streptococci (GBS). Although the colonization rate of Korean women by GBS is considered lower than in other countries, recent data on the maternal colonization rate of GBS are sparse. METHODS: From August 2008 to June 2009, swab specimens from the anorectus, vagina, and urethral orifice of a sample of 234 pregnant Korean women were placed in new Granada medium (NGM-H), tube medium (NGM-T), commercial NGM (NGM-B), and selective Todd-Hewitt broth (S-THB) for 18~24 hours in 5% CO2 at 35degrees C. Agar dilutional antimicrobial susceptibility tests, serotyping, and PCR were performed for GBS isolates. RESULTS: The colonization rate of GBS in pregnant women was 11.5% (27/234). Of the specimen cultures, 9.8% of anorectal cultures were positive, 8.1% of urethral orifice cultures were positive, and 7.3% of vagina cultures were positive. The detection rate of GBS in the different culture media was S-THB (96.3%), NGM-B (92.6%), NGM-H (88.9%), and NGM-T (85.2%). The distribution of GBS serotypes was as follows: III (29.6%), V and VI (22.2%), Ib and II (11.1%), and Ia (3.7%). 33.3% of GBS isolates were resistant to erythromycin and 44.4% to clindamycin. Among the nine erythromycin-resistant isolates, eight were serotype V and VI, which are erm(B) positive serotypes. CONCLUSION: The colonization of pregnant women by GBS, and the incidence of resistance of the GBS isolates to erythromycin and clindamycin were higher than those previously reported. Serotypes V and VI, GBS serotypes that carry the erm(B), are novel serotypes that have not previously been identified in pregnant Korean women.
