Effect of Alpha Adrenergic Drug on Serotonin Release in Rat Hippocampal Slices.
- Author:
Yun Seob SONG
1
;
Yong Soon YIM
;
Byoung Wook SEO
;
Min Eui KIM
;
Young Ho PARK
;
Moo Sang LEE
;
Hyung Ki CHOI
;
Young Soo AHN
Author Information
1. Department of Urology, College of Medicine, Soonchunhyang University, Korea.
- Publication Type:Original Article
- Keywords:
Alpha adrenergic drug;
Serotonin;
Hippocampus
- MeSH:
Adrenergic Agents;
Animals;
Brain;
Clonidine;
Epinephrine;
Hippocampus;
Humans;
Male;
Nervous System;
Neurotransmitter Agents;
Penile Erection;
Phenylephrine;
Radioactivity;
Rats*;
Receptors, Adrenergic;
Serotonin*
- From:Korean Journal of Urology
1999;40(9):1207-1212
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Peripheral adrenergic nervous system controls the penile erection and alpha adrenoceptors greatly outnumber beta adrenoceptors. It is known that adrenaline has a facilitatory effect but serotonin has an inhibitory effect on sexual drive centrally. But few about the effect of central alpha adrenergic drug to the release of serotonin centrally are known. Major adrenergic and serotoninergic innervation is found at the hippocampus. This study was performed to investigate the effect of alpha adrenergic drugs on serotonergic nervous system in rat hippocampal slices. MATERIALS AND METHODS: The hippocampus from the male rat brain was sliced. After 30 minutes` preincubation in the normal buffer, the slices were incubated for 20 minutes in a buffer containing 0.1 microM [3H]5-hydroxytryptamine(5-HT) for uptake, and washed. After administration of phenylephrine or clonidine, the release of [3H]5-HT into the buffer was measured and the radioactivities in each buffer and the tissue were counted and the results were expressed as a percentage of the total activity. The value of released [3H]5-HT was expressed as percent of the value at 50 minutes when a steady state of [3H]5-HT release was obtained. RESULTS: After administration of phenylephrine(10-5 M), clonidine(10-5 M), the values(mean+/-SE) were 133.4+/-7.3, 103.0+/-0.3 at 60 minutes and 128.8+/-4.6, 100.4+/-4.1(%) at 70 minutes compared to the values of control group, 96.6+/-1.9 % at 60 minutes and 89.6+/-2.3% at 70 minutes. The release of serotonin was increased significantly after administration of phenylephrine. CONCLUSIONS: It is suggested that alpha-1 adrenergic drugs may increase the release of serotonin and therefore the integration of these two neurotransmitters may be considered.
