Separation of Human Epidermal Cells by Using Percoll.
- Author:
Woo Young SIM
;
Eul Nam HAN
;
Nack In KIM
;
Choong Rim HAW
- Publication Type:Original Article
- Keywords:
Percoll;
Separation of human epidermal cells;
Density gradient1
- MeSH:
Epidermis;
Humans*;
Keratinocytes
- From:Korean Journal of Dermatology
1986;24(1):1-7
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The epidermis, which is composed mainly of keratinocyt;es, undergoes continual proliferation and differentiation, To study keratinocyte differentiation, it is necessary to separate the keratinocytes at their sucessive stages of maturation. We separate the keratinocytes by layers by using Percoll and obtain the relatively pure fractions of basal cells, spinous cells, and granular cells, 76. 8%, 67. 2%, 30%. respectively.
