Evaluation of the Automated Blood Bank Systems Galileo NEO and QWALYS-3 for ABO-RhD Typing and Antibody Screening.
- Author:
Mi Ae JANG
1
;
Jong Won OH
;
Seung Tae LEE
;
Ji Young SEO
;
Dae Won KIM
Author Information
1. Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. dwsmc.kim@samsung.com
- Publication Type:Original Article
- Keywords:
Galileo NEO;
QWALYS-3;
ABO-RhD typing;
Antibody screening;
Automation
- MeSH:
Automation;
Blood Banks*;
Mass Screening*
- From:Korean Journal of Blood Transfusion
2014;25(3):235-242
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: An automation system for ABO-RhD typing and antibody screening has been developed and its use is increasing. We compared the results of ABO-RhD typing and antibody screening tests using the manual (ABO-RhD typing) or semiautomated (antibody screening) method and with the automation instruments Galileo NEO (Immucor Gamma, Norcoss, USA) and QWALYS-3 (DIAGAST, Loos Cedex, France). METHODS: A total of 332 blood samples were tested for ABO-RhD typing in comparison with routine manual tests, and 236 samples for antibody screening in comparison with DS-Screening II (Bio-Rad Laboratories, 1785 Cressier FR, Switzerland). We evaluated the performance of Galileo NEO and QWALYS-3 in terms of concordance, carryover, and sensitivity test for ABO-RhD typing and antibody screening. RESULTS: The concordance rates of ABO-RhD typing results between the manual methods and the two instruments were 99.4% for Galileo NEO and 99.1% for QWALYS-3, respectively. On antibody screening tests, a concordance rate of 97.9% was observed between the semiautomated method and Galileo NEO or QWALYS-3, because of discordance in five specimens. The carryover was not observed for ABO-RhD typing and antibody screening. The overall sensitivity of the two automation instruments appears to be parallel with that of DS-Screening II except for anti-E. CONCLUSION: The Galileo NEO and QWALYS-3 system showed good performance, it can be used with confidence for routine pre-transfusion testing in the blood bank.
