Age-related Alterations of Bcl-2, Bax and Apoptosis in Ischemic-reperfused Rat Tibialis Anterior Muscles.
10.11637/kjpa.2003.16.2.97
- Author:
Youn Kyoung SEO
1
;
Jong Heon KIM
;
Hyun Sung LEEM
;
Chae Soo SHIN
;
Doo Jin PAIK
Author Information
1. Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, Korea. paikdj@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Ischemia;
Tibialis anterior muscle;
Bcl-2;
Bax;
Apoptosis
- MeSH:
Animals;
Apoptosis*;
Cell Death;
Humans;
Iliac Artery;
In Situ Nick-End Labeling;
Ischemia;
Male;
Muscle, Skeletal;
Muscles*;
Paraffin;
Rats*;
Rats, Sprague-Dawley;
Reperfusion
- From:Korean Journal of Physical Anthropology
2003;16(2):97-107
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Prolonged ischemic-reperfusion induces cellular damages and apoptosis in rat skeletal muscle. Such injury takes place in the phase of reperfusion following ischemia induction in part via regulating of apoptosis-related gene inductions. Among apoptosis-related gene products, Bcl-2 and Bax regulate the apoptotic response by inhibiting and promoting cell death, respectively. The present study was performed to examine the age-related alertation of expression of Bcl-2 and Bax along with apoptosis in rat tibialis anterior muscles following ischemia-reperfusion. 9 weeks, 30 weeks and more than 65 weeks old male Sprague-Dawley rats were divided into 2 groups such as controls, 4 hour ischemia. 4 hour ischemia group was divided into 3 subgroups based on reperfusion time. For ischemia, left commom iliac artery was occulded for 4 hours. The tibialis anterior muscles were removed 0, 3, and 24 hours after onset of reperfusion. Muscle samples were embeded in paraffin and 6 micrometer sections were made. The expression level of Bcl-2 and Bax were examed using immunohistochemical methods and apoptotic reactions was detected using TUNEL methods. The results obtained were as follows; 1. In control group, Bcl-2 and Bax were weakly immunostained in 9 weeks old rat tibialis anterior muscles. In 30 and 65 weeks old rat tibialis anterior muscles, Bcl-2 immunostaning was minimal. The level of Bax staining was increased in 30 and 65 weeks old rat tibialis anterior muscles, compared with those of 9 weeks old rats. The level of apopto-sis among different age groups were not altered. 2. In the 4 hour ischemia group, the level of Bax staining was correlated with the level of apoptosis in 9 weeks, 30 weeks and 65 weeks old rat tibialis anterior muscles. 3. The level of apoptosis was increased in older groups relative to younger groups in 4 hour ischemia. These results suggested that the level of apoptosis induced by ischemic-reperfusion in tibialis anterior muscle increase with ageing in rat tibialis anterior muscles, and that a increase of Bax expression is related to increased apoptosis.
