Human Sperm Morphology Comparison after Pre-and Post Percoll Gradient Centrifugation.
- Author:
Hyu KIM
;
Young Duk KIM
;
Seuk Ju LEE
;
Bo Young BANG
;
Hyun Jin SONG
- Publication Type:Original Article
- Keywords:
Percoll gradient centrifugation ( PGC );
Sperm morphology
- MeSH:
Centrifugation*;
Equidae;
Head;
Humans*;
Immersion;
Incheon;
Infertility;
Neck;
Semen;
Spermatozoa*
- From:Korean Journal of Obstetrics and Gynecology
1997;40(8):1581-1585
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to assess the efficiency of Percoll gradient centrifugation(PGC) as a method of sperm selection, we have examined morphological characteristics of spermatozoa from 40 teratozoospermic patients attending the Infertility Clinic of Inchon Gil Gerneral Hospital. Patients were divided into three groups according to percentage normal morphology in the fresh sample : group A(n=5), < 5% normal morphology : group B(n=21), 5~14% normal morphology : and group C(n=14), > 14% normal morphology. Morphology slides were perpared using Diff-Quik staining techniques and evaulated by Kruger strict criteria, under oil immersion at a magnification of X 1000, specific defects, head, neck and tail were assesed individualy. The results were as follows. 1. Following PGC, sperm samples with enhanced morphology were recovered for all groups. 2. For group A, PGC did not select a sample with significantly improved morphological characteristics. 3. Usually, sperm defects affected by PGC was head and neck. No significant difference was found for tail abnormality. In conclusion, Percoll gradient centrifugation is an efficient sperm preparation technique when the semen sample exhibits teratozoospermia, especially head or neck abnormality. However, in sample with < 5% normal form or tail abnormality, There is not significant improvement following PGC.
