The Influence of Different Concentrations of Cryoprotectants on Neuronal Cell Viability.
- Author:
Young Soo KIM
1
;
Ki Soo HAN
;
Uhn LEE
;
Young Bo KIM
;
Young Mi YOO
Author Information
1. Department of Neurosurgery, Ghil General Hospital, Inchon, Korea.
- Publication Type:Original Article
- Keywords:
Rat fetal neural tissue;
Cryopreservation;
Cell viability;
Cryoprotectant
- MeSH:
Animals;
Brain;
Cell Count;
Cell Survival*;
Cryopreservation;
Dimethyl Sulfoxide;
Neurodegenerative Diseases;
Neurons*;
Parkinson Disease;
Rats;
Tissue Survival
- From:Journal of Korean Neurosurgical Society
1998;27(3):299-304
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Apromising technique for the treatment of Parkinson's disease and other various neurodegenerative disorders is the transplantation of fetal neural tissue. There must, however, be a prompt and reliable source, and one solution is cryopreservation, where tissue viability can maintained for prolonged periods. Fetal neural tissue is, however, known to be susceptible to freeze-storage damage during cryopreservation. In this study, we examined the influence of different concentrations of cryoprotectants upon the survival of rat fetal neurones. Fetal rat brain tissue was frozen with 7-15% dimethyl sulfoxide(DMSO) and 10-50% fetal bovine serum(FBS) as cryoprotectants, then stored for a period of 5 months. Post-storage neuronal cell viabilty was assessed by vital staining followed by determination of cell density. Average total viability of frozen cells with 7% DMSO and 10-50% FBS was less than 50%. Cryopreserved cells with 10-50% DMSO and 10-50% FBS showed almost the same viability(around 70%). The highest viability was obtained with 15% DMSO+20% FBS combination(76%) and 10% DMSO+10% FBS combination(75%). Theoretically, the higher the concentration of cryoprotectants, the higher the viability: however, the best result was achieved stated above, when the combination of cryoprotectants was at the concentrations stated above.
