Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes.
- Author:
Yong Seung SHIN
1
;
Eung Goo LEE
;
Tae Sung JUNG
Author Information
1. Institue of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea. jungts@nongae.gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
antigenic protein;
bovine immunoglobulin classes;
immunoblotting;
Neospora caninum
- MeSH:
Animals;
Antigens, Protozoan/immunology;
Cattle;
Cattle Diseases/diagnosis/immunology/*parasitology;
Coccidiosis/diagnosis/immunology/parasitology/*veterinary;
Female;
Immunoblotting;
Immunoglobulin Idiotypes/diagnostic use/*immunology;
Neospora/*immunology
- From:Journal of Veterinary Science
2005;6(2):157-160
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study was attempted to compare the Neospora caninum (N. caninum) antigenic bands recognized by different bovine immunoglobulin classes. A total 10, 5, 2, and 6 antigenic bands were exhibited on immunoblot profiles against bovine IgM, IgE, IgA, and IgG, respectively. A 46 kDa band was probed as a common antigenic band except IgA; 69 kDa band was bovine IgM and IgE; 33, 37, 55, and 79 kDa bands were bovine IgM and IgG; 72 kDa band was found IgM and IgA profiles. Based on the analysis, it appeared that different immunoglobulin classes recognizing different antigenic molecules were cooperating to cope with neosporosis.
