Mutational Analysis of Caspase-7 and 8 Genes in Non-small Cell Lung Cancer.
- Author:
Young Hwa SOUNG
1
;
Jong Woo LEE
;
Seok Whan MOON
;
Keon Hyon JO
;
Young Pil WANG
;
Su Young KIM
;
Suk Woo NAM
;
Won Sang PARK
;
Jung Young LEE
;
Nam Jin YOO
;
Sug Hyung LEE
Author Information
1. Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea. suhulee@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Non-small cell lung cancer;
Caspase-7;
Caspase-8;
Apoptosis;
Mutation
- MeSH:
Apoptosis;
Carcinoma, Non-Small-Cell Lung*;
Caspase 7*;
Caspase 8;
Caspases;
Clinical Coding;
Humans;
Polymerase Chain Reaction
- From:Journal of Lung Cancer
2005;4(1):38-41
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE : Several lines of evidence have indicated that deregulation of apoptosis is involved in the mechanism of cancer development. Caspase-8 activation plays a central role in the initiation phase of apoptosis, while caspase-7 is one of the main execution phase caspases of apoptosis. The aim of this study was to explore the possibility that genetic alterations of the caspase-8 and caspase-7 genes are involved in the development of human non-small cell lung cancer (NSCLC). MATERIALS AND METHODS : We have analyzed the entire coding region of both the caspase-7 and caspase-8 genes to detect the somatic mutations in 100 NSCLCs by using polymerase chain reaction (PCR)- single strand conformation polymorphism (SSCP). RESULTS : The PCR-SSCP analysis detected no mutations in the entire coding regions of both the caspase-7 and caspase-8 genes in the NSCLCs. CONCLUSION : The data presented here suggests that both the caspase-7 and caspase-8 genes may not be somatically mutated in human NSCLCs
