Apoptosis Gene Expression Pattern Analysis of Jurkat Cells Treated with FK506.
10.4174/jkss.2009.77.4.225
- Author:
Tae Young JANG
1
;
Jae Sook LEE
;
Go Woon WOO
;
Hyun Chul KIM
;
Ho Kyun LEE
;
Sang Young CHUNG
Author Information
1. Department of Surgery, Hanil Hospital, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Tacrolimus;
Gene expression;
Apoptosis;
Jurkat cell
- MeSH:
Apoptosis;
Autoimmune Diseases;
Bone Marrow;
Calcineurin;
Cell Cycle;
Cell Cycle Checkpoints;
Cell Movement;
Cell Survival;
Gene Expression;
Gene Expression Profiling;
GTP-Binding Proteins;
Guanidines;
Humans;
Jurkat Cells;
Models, Theoretical;
Oligonucleotide Array Sequence Analysis;
Organ Transplantation;
Phenols;
Receptor, Insulin;
RNA;
Sp3 Transcription Factor;
T-Lymphocytes;
Tacrolimus;
Transplants
- From:Journal of the Korean Surgical Society
2009;77(4):225-237
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: FK506 (tacrolimus) is a widely used immunosuppressive agent in the treatment of various medical conditions, including autoimmune disease, bone marrow and organ transplantations. Previously FK506 was known to cause apoptotic death of human Jurkat T cells. METHODS: The current study was designed to analyze the gene expression pattern of Jurkat T cells after FK506 application by using cDNA microarray. Treatment of Jurkat T cells with FK506 resulted in a decrease of cell viability in a time- and dose-dependent manner. Next, total RNA of Jurkat T cells was extracted by using TRIzol reagent and used to carry out a confirmation test for the purity and integrity of total RNA. RESULTS: Gene expression levels related to apoptosis and cell cycle process were mainly focused to analyze in FK506-treated Jurkat T cells. According to the inhibition of calcineurin activity, MARCKS in PKC substrates and Sp3 transcription factor was markedly increased in FK506-treated cells. Also, cell cycle control gene Id1 and Id3 were induced in expression from FK506-treated Jurkat T cells. However, FK506 decreased the expression of Src homology 2, G protein, and MEK 2 genes in bioactive peptide induced signaling pathway. It also reduced the expression level of the insulin receptor, DRPLA and Bai1-associated protein 2 genes, which are involved in the regulation of cell motility and morphology control. CONCLUSION: The author will continue to pursue the exact functional roles of genes that are markedly changed in expression by FK506 in human Jurkat T cells in vitro and in vivo experimental models.
