Molecular Identification of Taenia Tapeworms by Cox1 Gene in Koh Kong, Cambodia.
10.3347/kjp.2011.49.2.195
- Author:
Hyeong Kyu JEON
1
;
Tai Soon YONG
;
Woon Mok SOHN
;
Jong Yil CHAI
;
Sung Jong HONG
;
Eun Taek HAN
;
Hoo Gn JEONG
;
Tep CHHAKDA
;
Muth SINUON
;
Duong SOCHEAT
;
Keeseon S EOM
Author Information
1. Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine, Cheongju 361-711, Korea. kseom@chungbuk.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Taenia saginata;
Taenia solium;
molecular survey;
egg DNA;
multiplex PCR
- MeSH:
Adolescent;
Adult;
Animals;
Cambodia;
Child;
Cyclooxygenase 1/*genetics;
DNA Primers/genetics;
DNA, Helminth/chemistry/genetics;
Feces/parasitology;
Female;
Helminth Proteins/*genetics;
Humans;
Male;
Middle Aged;
Polymerase Chain Reaction;
Sequence Analysis, DNA;
Taenia saginata/*enzymology/*genetics/isolation & purification;
Taenia solium/*enzymology/*genetics/isolation & purification;
Taeniasis/*parasitology;
Young Adult
- From:The Korean Journal of Parasitology
2011;49(2):195-197
- CountryRepublic of Korea
- Language:English
-
Abstract:
We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.
