Effect of Mitomycin C on the Proliferation and Nitric Oxide Production in the Cultured Trabecular Meshwork Cells.
- Author:
Beum Joon CHO
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, School of Medicine, Catholic University of Daegu, Korea. jwkim@cataegu.ac.kr
- Publication Type:Original Article
- Keywords:
Culture;
Griess reaction;
Mitomycin C;
MTT;
Nitric oxide;
Proliferation;
Trabecular meshwork cells
- MeSH:
Humans;
Mitomycin*;
Nitric Oxide*;
Tissue Donors;
Trabecular Meshwork*
- From:Journal of the Korean Ophthalmological Society
2003;44(2):485-490
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of mitomycin C on the proliferation of cultured trabecular cells and its relation to nitric oxide (NO) production. METHODS: The effect of NO donor, SIN-1, on the proliferation of primarily cultured porcine trabecular meshwork cells was studied with MTT assay. After treatment with mitomycin C at various concentrations for 1 hour, the proliferation was assessed by MTT assay and the production of nitrite was assessed by Griess reaction after 24 hours and 3 days respectively. RESULTS: SIN-1 significantly inhibited proliferation of cultured trabecular meshwork cells (p<0.05). Mitomycin C also suppressed the cellular proliferation(p<0.05) but did not affect the production of nitrite (p>0.05). CONCLUSIONS: Both NO donor and mitomycin C have an inhibitory effect on the proliferation of trabecular meshwork cells. However, inhibitory effect of mitomycin C was not related to the production of NO.
