The Neuroprotective Effect of Intravitreally Injected CNTF on Rat Retinal Ganglion Cell in Optic Nerve Crush Injury Model.
- Author:
Ja Heon KANG
1
;
Ki Ho PARK
;
Dong Myung KIM
Author Information
1. Department of Ophthalmology, Seoul Paik Hospital, Inje University, Korea.
- Publication Type:Original Article
- Keywords:
Ciliary neurotrophic factor (CNTF);
Optic nerve crush injury;
Retinal ganglion cell;
Neuroprotection
- MeSH:
Aneurysm;
Animals;
Ciliary Neurotrophic Factor*;
Dextrans;
Fluorescence;
Neuroprotective Agents*;
Optic Nerve Injuries;
Optic Nerve*;
Rats*;
Rats, Sprague-Dawley;
Retina;
Retinal Ganglion Cells*;
Retinaldehyde*
- From:Journal of the Korean Ophthalmological Society
2003;44(2):491-495
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To make an optic nerve crush injury model and to investigate the neuroprotective effect of intravitreally injected ciliary neurotrophic factor (CNTF) on rat retinal ganglion cells (RGCs) in the model. METHODS: The optic nerves of 12 Sprague-Dawley rats were crushed at 3 mm posterior to the eyeball for 1 minute using aneurysm clip (110 g). Two micrograms of CNTF in 2 micro liter of vehicle was injected intravitreally in one group (n=6) and 2 micro liter of PBS was injected in the control group (n=6) at 4, 7, and 10 days after the optic nerve injury. After 2 weeks, the retrograde labeling of the RGCs was done by the dextran tetramethylrhodamine. Twenty-four hours after the labeling, the retina was wholly mounted and the labeled RGCs were counted under the fluorescence microscope. RESULTS: The death of RGCs in this model began at 1 week and continued for 3 weeks. The number of labeled RGCs in CNTF-injected group (510+/-139/mm2) were significantly higher than that in control group (345+/-87/mm2)(p<0.05). CONCLUSIONS: The optic nerve crush injury model was established by use of aneurysm clip. In this model, the intravitreally injected CNTF had a neuroprotective effect on the rat RGCs.
