Effect of Gelrite on the Proliferation of Cultured Human Tenon's Capsule Fibroblasts.
- Author:
Jae Woo KIM
1
;
Keun Hae KIM
;
Yong Hun JIN
Author Information
1. Department of Ophthalmology, School of Medicine, Catholic University of Daegu, Korea. jwkim@cataegu.ac.kr
- Publication Type:Original Article
- Keywords:
Culture;
Fibroblast;
Gelrite;
MTT;
Proliferation
- MeSH:
Benzalkonium Compounds;
Fibroblasts*;
Humans*;
Tenon Capsule*
- From:Journal of the Korean Ophthalmological Society
2003;44(2):496-501
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of Gelrite (Gelrite gellan gum), a natural gelling agent, on the viability and proliferation of human Tenon's capsule fibroblasts in tissue culture. METHODS: We studied the effect of two common preservatives, Gelrite and pure benzalkonium chloride (BAK) on the proliferation of primarily cultured human Tenon's capsule fibroblasts. After treatment of Gelrite and BAK with various serial dilutions, proliferation was assessed by MTT assay and the morphologic changes were evaluated with by inverted phase contrast microscopic examination after 24 hours and 1 week respectively. RESULTS: Gelrite did not affect the proliferation of fibroblasts and revealed no apparent morphologic change or viability of the fibroblasts in tissue culture. Also BAK did not affect the proliferation but inhibited the proliferation of the fibroblasts with toxic effects on cell morphology. CONCLUSIONS: Gelrite showed no apparent effect on the proliferation and morphology of the primarily cultured human Tenon's capsule fibroblasts.
