Cell Death of Corneal Fibroblasts Induced by Tumor Necrosis Factor-alphaand Interferon-gamma.
- Author:
Yong Myeong KIM
1
;
Hyung Jin KOO
;
Eun Young CHO
;
Eui Sang CHUNG
;
Woo Jung KIM
Author Information
1. Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cell death;
Corneal fibroblasts;
INF-gamma;
TNF-alpha
- MeSH:
Cell Death*;
Cell Survival;
Cornea;
Cytokines;
Fibroblasts*;
Humans;
Interferon-gamma*;
Necrosis*;
Trypan Blue;
Tumor Necrosis Factor-alpha
- From:Journal of the Korean Ophthalmological Society
2001;42(6):852-856
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the effect in the cell death of corneal fibroblasts when TNF-alphaand INF-gamma were given together. METHODS: Fibroblasts harvested from the human cornea were cultured in DMEM, then, nothing(control: Group 1), TNF-alphaonly(50 ng/ml : Group 2), INF-gammaonly(1.0 x 10(3)u/ml : Group 3), and a combination of both(Group 4) were added. We assessed the cell viability of the each group by the trypan blue exclusion assay at 4, 8, 12, 24, 48 hours after addition of cytokines. RESULTS: The cell viability at 48 hour after treatment was 94.27% in group 1, 90.68%(p=0.09) in group 2, 93.31%(p=0.45) in group3, and there was no statistical difference among the groups. Statistically signi-ficant decrease of the cell viability was achieved in group 4(82.86%, p=0.002). CONCLUSIONS: Cell death of human corneal fibroblasts had been increased after treatment with a combination of TNF-alphaand INF-gamma. These findings suggest that there could be some kind of interaction among the cytokines.
