Inhibitory Effect of Genistein on the Proliferation of Lens Epithelial Cells.
- Author:
Kui Doo BYUN
1
;
Young Soo YUN
;
Jung Hyub OH
Author Information
1. Department of Ophthalmology, College of Medicine, Inha University, Inchon, Korea.
- Publication Type:Original Article
- Keywords:
Lens epithelial cells;
Genistein;
Growth factors
- MeSH:
Anterior Chamber;
Cell Proliferation;
Endothelium, Corneal;
Epidermal Growth Factor;
Epithelial Cells*;
Fibronectins;
Genistein*;
Intercellular Signaling Peptides and Proteins;
Perfusion;
Phosphorylation;
Spectrophotometry;
Transforming Growth Factor beta
- From:Journal of the Korean Ophthalmological Society
2001;42(6):877-888
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The objective of the present studies was to evaluate the effect of genistein on inhibiting growth factor-induced proliferation of lens epithelial cells. METHODS: The effect of genistein on cell proliferation was assayed in cultured pig lens epithelial cells after combined treatment of genistein with several growth factors including bFGF, TGF-beta, EGF and PDGF. The analysis of cell proliferation was done using spectrophotometry. To verify the inhibitory influence of genistein on the proliferation of LECs from the molecular biologic level, we determined using western blotting(mRNA of fibronectin). Also, the present study was aimed to evaluate alterations of the function and the ultrastructure of rabbit corneal endothelium following perfusion of the anterior chamber with balanced salt solution(BSS) or genistein in vivo. RESULTS: Each growth factor stimulated lens epithelial cell proliferation effectively after 24 hours of culture. Gradual reduction of lens epithelial cell density occurred as the concentration of genistein increased and the degree of reduction was the most prominent at 100 micrometer of genistein. Marked reduction of ERK phosphorylation and the expression of fibronectin were noted in the groups treated with bFGF and genistein compared with the groups treated with bFGF alone. There was no significant difference in corneal thickness between the BSS and the genistein treated groups in vivo. CONCLUSIONS: Genistein at the concentration of 100 micrometer effectively suppressed the proliferating activity of growth factors on the LECs and showed no toxicity to the corneal endothelium.
