Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers.
10.4142/jvs.2015.16.4.423
- Author:
Huayuan LIN
1
;
Qiqi HUANG
;
Xiaoquan GUO
;
Ping LIU
;
Weilian LIU
;
Yuelong ZOU
;
Shuliang ZHU
;
Guangfu DENG
;
Jun KUANG
;
Caiying ZHANG
;
Huabin CAO
;
Guoliang HU
Author Information
1. Clinical Veterinary Laboratory, College of Animal Science and Technology, Jiangxi Agricultural University, Jiangxi 330029, China. Xqguo20720@aliyun.com
- Publication Type:Original Article
- Keywords:
antioxidant;
growing layers;
hyperuricemia;
nephropathogenic infectious bronchitis virus;
xanthine oxidase
- MeSH:
Antioxidants;
Chickens;
Glutathione Peroxidase;
Hyperuricemia;
Infectious bronchitis virus*;
Kidney;
Malondialdehyde;
RNA, Messenger*;
Superoxide Dismutase;
Uric Acid;
Xanthine Oxidase*;
Xanthine*
- From:Journal of Veterinary Science
2015;16(4):423-429
- CountryRepublic of Korea
- Language:English
-
Abstract:
To assess relationships between xanthine oxidase (XOD) and nephropathogenic infectious bronchitis virus (NIBV) infection, 240 growing layers (35 days old) were randomly divided into two groups (infected and control) of 120 chickens each. Each chicken in the control and infected group was intranasally inoculated with 0.2 mL sterile physiological saline and virus, respectively, after which serum antioxidant parameters and renal XOD mRNA expression in growing layers were evaluated at 8, 15 and 22 days post-inoculation (dpi). The results showed that serum glutathione peroxidase and superoxide dismutase activities in the infected group were significantly lower than in the control group at 8 and 15 dpi (p < 0.01), while serum malondialdehyde concentrations were significantly higher (p < 0.01). The serum uric acid was significantly higher than that of the control group at 15 dpi (p < 0.01). In addition, the kidney mRNA transcript level and serum activity of XOD in the infected group was significantly higher than that of the control group at 8, 15 and 22 dpi (p < 0.05). The results indicated that NIBV infection could cause the increases of renal XOD gene transcription and serum XOD activity, leading to hyperuricemia and reduction of antioxidants in the body.
