Profiling of antimicrobial resistance and plasmid replicon types in beta-lactamase producing Escherichia coli isolated from Korean beef cattle.
10.4142/jvs.2015.16.4.483
- Author:
Seung Won SHIN
1
;
Myunghwan JUNG
;
Min Kyung SHIN
;
Han Sang YOO
Author Information
1. Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. yoohs@snu.ac.kr
- Publication Type:Original Article
- Keywords:
beta-lactamase;
antimicrobial resistance;
Escherichia coli;
plasmid replicon typing
- MeSH:
Amoxicillin;
Ampicillin;
Animals;
beta-Lactamases*;
Cattle*;
Cefotaxime;
Cefoxitin;
Ceftazidime;
Cephalothin;
Diffusion;
Escherichia coli*;
Escherichia*;
Humans;
Korea;
Livestock;
Plasmids*;
Replicon*;
Sequence Analysis, DNA;
Tissue Donors
- From:Journal of Veterinary Science
2015;16(4):483-489
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, 78 isolates of Escherichia coli isolated from Korean beef cattle farms were investigated for the production of extended-spectrum beta-lactamase (ESBL) and/or AmpC beta-lactamase. In the disc diffusion test with ampicillin, amoxicillin, cephalothin, ceftiofur, cefotaxime, ceftazidime, and cefoxitin, 38.5% of the isolates showed resistance to all of ampicillin, amoxicillin, and cephalothin. The double disc synergy method revealed that none of the isolates produced ESBL or AmpC beta-lactamases. DNA sequencing showed that all isolates encoded genes for TEM-1-type beta-lactamase. Moreover, 78.2% of the isolates transferred the TEM-1-type beta-lactamase gene via conjugation. In plasmid replicon typing of all donors, IncFIB and IncFIA were identified in 71.4% and 41.0% of plasmids, respectively. In transconjugants, IncFIB and IncFIA were the most frequent types detected (61.5% and 41.0%, respectively). Overall, the present study indicates that selection pressures of antimicrobials on beta-lactamases in beef cattle may be low relative to other livestock animals in Korea. Moreover, to reduce selection pressure and dissemination of beta-lactamase, the long-term surveillance of antimicrobial use in domestic beef cattle should be established.
