Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system.
10.4142/jvs.2015.16.4.509
- Author:
Wonyou LEE
1
;
Kilyoung SONG
;
Inhyung LEE
;
Hyungdo SHIN
;
Byeong Chun LEE
;
Seongchan YEON
;
Goo JANG
Author Information
1. Department of Theriogenology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea. snujang@snu.ac.kr
- Publication Type:Original Article
- Keywords:
cloned horse;
embryo transfer;
follicle aspiration;
short needle;
somatic cell nuclear transfer
- MeSH:
Clone Cells*;
DNA;
DNA, Mitochondrial;
Embryo Transfer;
Embryology;
Embryonic Structures;
Horses*;
Humans;
Mental Competency;
Microsatellite Repeats;
Needles*;
Oocyte Retrieval;
Oocytes*;
Tissue Donors
- From:Journal of Veterinary Science
2015;16(4):509-516
- CountryRepublic of Korea
- Language:English
-
Abstract:
Transvaginal ultrasound-guided follicle aspiration is one method of obtaining recipient oocytes for equine somatic cell nuclear transfer (SCNT). This study was conducted: (1) to evaluate the possibility of oocyte aspiration from pre-ovulatory follicles using a short disposable needle system (14-G) by comparing the oocyte recovery rate with that of a long double lumen needle (12-G); (2) to investigate the developmental competence of recovered oocytes after SCNT and embryo transfer. The recovery rates with the short disposable needle vs. the long needle were not significantly different (47.5% and 35.0%, respectively). Twenty-six SCNT embryos were transferred to 13 mares, and one mare delivered a live offspring at Day 342. There was a perfect identity match between the cloned foal and the cell donor after analysis of microsatellite DNA, and the mitochondrial DNA of the cloned foal was identical with that of the oocyte donor. These results demonstrated that the short disposable needle system can be used to recover oocytes to use as cytoplasts for SCNT, in the production of cloned foals and for other applications in equine embryology
