Rosmarinic Acid Attenuates Cell Damage against UVB Radiation-Induced Oxidative Stress via Enhancing Antioxidant Effects in Human HaCaT Cells.
10.4062/biomolther.2015.069
- Author:
Pattage Madushan Dilhara Jayatissa FERNANDO
1
;
Mei Jing PIAO
;
Kyoung Ah KANG
;
Yea Seong RYU
;
Susara Ruwan Kumara Madduma HEWAGE
;
Sung Wook CHAE
;
Jin Won HYUN
Author Information
1. Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea. jinwonh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Antioxidant system;
Oxidative stress;
Reactive oxygen species;
Rosmarinic acid;
Ultraviolet B
- MeSH:
Antioxidants*;
Catalase;
Cytoprotection;
DNA;
Heme Oxygenase-1;
Humans*;
Keratinocytes;
Oxidative Stress*;
Reactive Oxygen Species;
Skin Diseases;
Superoxide Dismutase;
Transcription Factors
- From:Biomolecules & Therapeutics
2016;24(1):75-84
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was designed to investigate the cytoprotective effect of rosmarinic acid (RA) on ultraviolet B (UVB)-induced oxidative stress in HaCaT keratinocytes. RA exerted a significant cytoprotective effect by scavenging intracellular ROS induced by UVB. RA also attenuated UVB-induced oxidative macromolecular damage, including protein carbonyl content, DNA strand breaks, and the level of 8-isoprostane. Furthermore, RA increased the expression and activity of superoxide dismutase, catalase, heme oxygenase-1, and their transcription factor Nrf2, which are decreased by UVB radiation. Collectively, these data indicate that RA can provide substantial cytoprotection against the adverse effects of UVB radiation by modulating cellular antioxidant systems, and has potential to be developed as a medical agent for ROS-induced skin diseases.
