Anti-Melanogenic Potentials of Nanoparticles from Calli of Resveratrol-Enriched Rice against UVB-Induced Hyperpigmentation in Guinea Pig Skin.
10.4062/biomolther.2015.165
- Author:
Taek Hwan LEE
1
;
Ji Hee KANG
;
Jae Ok SEO
;
So Hyeon BAEK
;
Sang Hyun MOH
;
Jae Kyoung CHAE
;
Yong Un PARK
;
Young Tag KO
;
Sun Yeou KIM
Author Information
1. College of Pharmacy, Yonsei University, Incheon 21983, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Plant tissue culture;
High-pressure homogenization;
Melanogenesis;
Resveratrol-enriched rice;
Nanoparticles
- MeSH:
Animals;
Epidermis;
Guinea Pigs*;
Guinea*;
Hydrogen-Ion Concentration;
Hyperpigmentation*;
Melanins;
Monophenol Monooxygenase;
Nanoparticles*;
Particle Size;
Plants;
Skin Pigmentation;
Skin*
- From:Biomolecules & Therapeutics
2016;24(1):85-93
- CountryRepublic of Korea
- Language:English
-
Abstract:
We already reported that genetically engineered resveratrol-enriched rice (RR) showed to down-regulate skin melanogenesis. To be developed to increase the bioactivity of RR using calli from plants, RR was adopted for mass production using plant tissue culture technologies. In addition, high-pressure homogenization (HPH) was used to increase the biocompatibility and penetration of the calli from RR into the skin. We aimed to develop anti-melanogenic agents incorporating calli of RR (cRR) and nanoparticles by high-pressure homogenization, examining the synergistic effects on the inhibition of UVB-induced hyperpigmentation. Depigmentation was observed following topical application of micro-cRR, nano-calli of normal rice (cNR), and nano-cRR to ultraviolet B (UVB)-stimulated hyperpigmented guinea pig dorsal skin. Colorimetric analysis, tyrosinase immunostaining, and Fontana-Masson staining for UVB-promoted melanin were performed. Nano-cRR inhibited changes in the melanin color index caused by UVB-promoted hyperpigmentation, and demonstrated stronger anti-melanogenic potential than micro-cRR. In epidermal skin, nano-cRR repressed UVB-promoted melanin granules, thereby suppressing hyperpigmentation. The UVB-enhanced, highly expressed tyrosinase in the basal layer of the epidermis was inhibited by nano-cRR more prominently than by micro-cRR and nano-cNR. The anti-melanogenic potency of nano-cRR also depended on pH and particle size. Nano-cRR shows promising potential to regulate skin pigmentation following UVB exposure.
