Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells).
10.4062/biomolther.2015.183
- Author:
Na Young LEE
1
;
Hyun Joo PARK
;
Young Sook KANG
Author Information
1. College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, Seoul 04310, Republic of Korea. yskang@sm.ac.kr
- Publication Type:Original Article
- Keywords:
Glucose uptake;
Bisphosphonates;
Inner blood-retinal barrier;
Retinal capillary endothelial cells;
Mevalonate pathway
- MeSH:
Alendronate;
Animals;
Blood-Retinal Barrier;
Capillaries*;
Cytochalasin B;
Diphosphonates*;
Endothelial Cells*;
Glucose*;
Hand;
Histamine;
Mevalonic Acid;
Rats*;
Retinaldehyde*
- From:Biomolecules & Therapeutics
2016;24(1):94-98
- CountryRepublic of Korea
- Language:English
-
Abstract:
The objective of the present study was to elucidate the effect of bisphosphonates, anti-osteoporosis agents, on glucose uptake in retinal capillary endothelial cells under normal and high glucose conditions. The change of glucose uptake by pre-treatment of bisphosphonates at the inner blood-retinal barrier (iBRB) was determined by measuring cellular uptake of [3H]3-O-methyl glucose (3-OMG) using a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB cells) under normal and high glucose conditions. [3H]3-OMG uptake was inhibited by simultaneous treatment of unlabeled D-glucose and 3-OMG as well as glucose transport inhibitor, cytochalasin B. On the other hand, simultaneous treatment of alendronate or pamidronate had no significant inhibitory effect on [3H]3-OMG uptake by TR-iBRB cells. Under high glucose condition of TR-iBRB cells, [3H]3-OMG uptake was increased at 48 h. However, [3H]3-OMG uptake was decreased significantly by pre-treatment of alendronate or pamidronate compared with the values for normal and high glucose conditions. Moreover, geranylgeraniol (GGOH), a mevalonate pathway intermediate, increased the uptake of [3H]3-OMG reduced by bisphosphonates pre-treatment. But, pre-treatment of histamine did not show significant inhibition of [3H]3-OMG uptake. The glucose uptake may be down regulated by inhibiting the mevalonate pathway with pre-treatment of bisphosphonates in TR-iBRB cells at high glucose condition.
