Involvement of protein kinase C pathway in UVC-stimulated phospholipase D2 activity in Vero 76 cells.
- Author:
Sungyeul KIM
1
;
Myung Sun CHOI
;
Myung Un CHOI
Author Information
1. School of Chemistry, Seoul National University, Seoul 151-747, Korea. muchoi@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
phospholipase D;
protein kinase C;
reactive oxygen species;
ultraviolet rays
- MeSH:
Animals;
Antioxidants/metabolism;
Calcium/metabolism;
Cercopithecus aethiops;
Chelating Agents/pharmacology;
Enzyme Activation/radiation effects;
Mice;
Phospholipase D/genetics/*metabolism;
Protein Isoforms/genetics/metabolism;
Protein Kinase C/*metabolism;
Protein Kinase Inhibitors/pharmacology;
Reactive Oxygen Species;
Research Support, Non-U.S. Gov't;
Signal Transduction/*radiation effects;
*Ultraviolet Rays;
Vero Cells
- From:Experimental & Molecular Medicine
2005;37(5):418-426
- CountryRepublic of Korea
- Language:English
-
Abstract:
Phospholipase D (PLD) activity is known to be related to oxidant-induced cellular signaling and membrane disturbance. Previously, an induction of PLD activity in various cell lines by X-ray irradiation was observed. In this study, we examined the effect of UVC radiation on the PLD activity in Vero 76 cells. At a dose of 10 kJ/m2 of UVC irradiation, the PLD activity was stimulated approximately 10-fold over the basal activity. This UVC-induced PLD activity was found to be dependent on the presence of extracellular calcium and was inhibited by catalase as well as amifostine-an intracellular thiol antioxidant. Pretreatments with Ro32-0432-a selective inhibitor of protein kinase C (PKC)-and downregulation of PKC by preincubation of phorbol 12-myristate 13-acetate significantly inhibited the UVC-induced PLD activity. UVC-stimulated PLD activity was observed only in murine PLD2 (mPLD2)-transfected Vero 76 cells and not in human PLD1 (hPLD1)-transfected cells. Transient incorporation of PKC with mPLD2 and the phosphorylation of mPLD2 by a and b forms of PKC by UVC irradiation were observed. These results suggest that the UVC-stimulated PLD activity in Vero 76 cells is mediated through transient phosphorylation of PLD2 by the translocation of PKC to PLD2.
