Up-regulation of defense enzymes is responsible for low reactive oxygen species in malignant prostate cancer cells.
- Author:
Hye Won LIM
1
;
Suntaek HONG
;
Wook JIN
;
Seunghwan LIM
;
Su Jung KIM
;
Hyun Jung KANG
;
Eun Hee PARK
;
Kisup AHN
;
Chang Jin LIM
Author Information
1. Division of Life Sciences, Kangwon National University, Chuncheon 200-701, Korea. cjlim@kangwon.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
catalase;
glutathione;
glutathione reductase;
glutathione S-transferase;
metastasis;
oxidative stress;
prostatic neoplasms;
thioredoxin
- MeSH:
Antioxidants/metabolism;
Cell Line, Tumor;
Enzyme Induction;
Gene Expression Regulation, Neoplastic;
Humans;
Male;
*Oxidative Stress;
Prostatic Neoplasms/enzymology/*genetics/*metabolism;
Reactive Oxygen Species/*metabolism;
Research Support, Non-U.S. Gov't;
Thioredoxin Reductase (NADPH)/metabolism;
Up-Regulation/*genetics
- From:Experimental & Molecular Medicine
2005;37(5):497-506
- CountryRepublic of Korea
- Language:English
-
Abstract:
Reactive oxygen species (ROS) are involved in a diversity of important phenomena in the process of tumor development. To investigate the alterations of oxidative stress and their related systems in tumor progression, a variety of components in the antioxidative stress defense system were examined in prostate cancer cell lines, PC3 and LNCaP. Cell surface molecules involved in metastasis were expressed highly in PC3 cells compared with LNCaP cells, and strong invasion ability was shown in PC3 cells only. ROS level in LNCaP cells was twice higher than that in PC3 cells, although nitric oxide (NO) level was similar between the two cell lines. The content of GSH increased up to about 2-fold in PC3 compared with LNCaP. Activities of glutathione reductase, thioredoxin reductase, and glutathione S-transferase except catalase are significantly higher in PC3 cells than in LNCaP cells. Furthermore, oxidative stress-inducing agents caused down-regulation of GSH and glutathione S-transferase much more significantly in LNCaP cells than in PC3 cells. These results imply that malignant tumor cells may maintain low ROS content by preserving relatively high anti-oxidative capacity, even in the presence of stressful agents.
