Analysis of extended-spectrum beta-lactamase TEM produced by Klebsiella Pneumoniae.
- Author:
Hyunjoo PAI
1
;
Jung Min KIM
;
Young Mi KWON
;
Jong Won KIM
Author Information
1. Division of Infectious Disease, University of Dankook Medical College, Chonan.
- Publication Type:Original Article
- Keywords:
extended-spectrum beta-lactamase;
TEM-52;
Klebsiella pneumoniae
- MeSH:
Amino Acids;
Aztreonam;
beta-Lactamases*;
Cefotaxime;
Ceftazidime;
Glutamic Acid;
Glycine;
Hydrolysis;
Isoelectric Point;
Klebsiella pneumoniae*;
Klebsiella*;
Korea;
Lysine;
Methionine;
Penicillinase;
Plasmids;
Pneumonia;
Polymerase Chain Reaction;
Prevalence;
Serine;
Threonine
- From:Korean Journal of Infectious Diseases
1998;30(2):126-133
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The prevalence of extended-spectrum beta-lactamase(ESBL)-producing organisms has been increasing in Korea. We performed a study to characterize various TEM derivatives with clinical isolates of Klebsiella pneumoniae collected from 3 hospitals in Korea. METHODS: Fifty-seven isolates of ESBL-producing K. pneumoniae collected from 3 hospitals were screened by TEM-specific PCR for the carriage of TEM genes. Thirteen strains were found to have TEM-related genes. Eleven blaTEM genes were amplifed and sequenced. The transfer of resistance was tested by conjugation and isoelectric points of beta-lactamases were determined. MICs were measured to obtain a resistance pattern for each indivudual wild- type and transconjugant strain. The hydrolysis rate of TEM-52 was measured spectrophotometrically. RESULTS: Ten strains carried plasmid-mediated CTEM-52 gene, which sequence showed the substitution of 3 amino acids compared to that of TEM-1: 104 glutamic acid --> lysine (GAG --> AAG), 182 methionine --> threonine (ATG --> ACG), and 238 glycine --> serine (GGT --> AGT). MIC showed that TEM-52 mediated a resistance to ceftazidime and aztreonam at a lower level than to cefotaxime. TEM-52 enzyme hydrolyzed cefotaxime efficiently (Vmax, 340) and showed fairly weak activity for ceftazidime (Vmax, 15.1), but very weak activity for aztreonam. The gene of TEM-52 beta-lactamases was mediated by approximately 77-kb plasmids. CONCLUSION: From these results, we conclude that the TEM-52 beta-lactamase is a common TEM- type ESBL in K. pneumoniae in Korea.
