beta-irradiation (166Ho patch)-induced skin injury in mini-pigs: effects on NF-kappaB and COX-2 expression in the skin.
- Author:
Joong Sun KIM
1
;
Kyung Jin RHIM
;
Won Seok JANG
;
Sun Joo LEE
;
Yeonghoon SON
;
Seung Sook LEE
;
Sunhoo PARK
;
Sang Moo LIM
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: beta-ray; irradiation; pig; skin
- MeSH: Animals; Cyclooxygenase 2/genetics/*metabolism; *Holmium; Male; NF-kappa B/genetics/*metabolism; Radiation Injuries, Experimental/metabolism/*veterinary; Skin/metabolism/*radiation effects; Swine; Swine, Miniature
- From:Journal of Veterinary Science 2015;16(1):1-9
- CountryRepublic of Korea
- Language:English
- Abstract: In the present study, the detrimental effect of beta-emission on pig skin was evaluated. Skin injury was modeled in mini-pigs by exposing the animals to 50 and 100 Gy of beta-emission delivered by 166Ho patches. Clinicopathological and immunohistochemical changes in exposed skin were monitored for 18 weeks after beta-irradiation. Radiation induced desquamation at 2~4 weeks and gradual repair of this damage was evident 6 weeks after irradiation. Changes in basal cell density and skin depth corresponded to clinically relevant changes. Skin thickness began to decrease 1 week after irradiation, and the skin was thinnest 4 weeks after irradiation. Skin thickness increased transiently during recovery from irradiation-induced skin injury, which was evident 6~8 weeks after irradiation. Epidermal expression of nuclear factor-kappa B (NF-kappaB) differed significantly between the untreated and irradiated areas. One week after irradiation, cyclooxygenase-2 (COX-2) expression was mostly limited to the basal cell layer and scattered among these cells. High levels of COX-2 expression were detected throughout the full depth of the skin 4 weeks after irradiation. These findings suggest that NF-kappaB and COX-2 play roles in epidermal cell regeneration following beta-irradiation of mini-pig skin.
