Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene.
- Author:
Yuqiang CHENG
1
;
Yingjie SUN
;
Hengan WANG
;
Shuduan SHI
;
Yaxian YAN
;
Jing LI
;
Chan DING
;
Jianhe SUN
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: duck; innate immune response; myeloid differentiation factor 88; Newcastle disease virus; Toll-like receptor 5
- MeSH: 2',5'-Oligoadenylate Synthetase/genetics/metabolism; Animals; Cell Line; *Cloning, Molecular; Ducks; Gene Expression Regulation/*physiology; Humans; Immunity, Innate; Myeloid Differentiation Factor 88/genetics/metabolism; Myxovirus Resistance Proteins/genetics/metabolism; Newcastle Disease/metabolism; Newcastle disease virus/classification; RNA, Messenger/genetics/metabolism; Species Specificity; Toll-Like Receptor 5/genetics/*metabolism
- From:Journal of Veterinary Science 2015;16(1):37-46
- CountryRepublic of Korea
- Language:English
- Abstract: Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2'-5'-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the bursa of Fabricius, spleen, trachea, lung, jejunum, rectum, and skin; moderately expressed in the muscular and glandular tissues, duodenum, ileum, caecum, and pancreas; and minimally expressed in the heart, liver, kidney, and muscle. DF-1 or HeLa cells transfected with DNA constructs encoding dTLR5 can activate NF-kappaB leading to the activation of interleukin-6 (IL-6) promoter. When we challenged ducks with a Herts33 Newcastle disease virus (NDV), mRNA transcription of the antiviral molecules Mx, Double stranded RNA activated protein kinase (PKR), and OAS was up-regulated in the liver, lung, and spleen 1 and 2 days post-inoculation.
