The change of cytological components of platelet mass prepared and stored in different conditions
- Author:
Son Truong Nguyen
- Publication Type:Journal Article
- Keywords:
platelet mass
- MeSH:
Blood Platelets;
Tissue Donors
- From:Journal of Medical and Pharmaceutical Information
1999;(12):30-34
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Random donor platelet concentrations (PCs) can be prepared by 2 methods: from platelet rich plasma (PRP) and from buffy coat (BC). In this study, the changes of cytological properties of the PCs prepared and stored at the different conditions were evaluated. Method: 45 samples of whole blood were divided into 3 groups: PCs-PRP stored at 22oC with shaking (n=15), PCs-BC stored at 22oC with shaking and PCs-PRP stored at 4oC without shaking. The assessment of platelet, red cell, white cell counts and hypotonic shock responses (HCR) were performed. Result: the yield of platelet separation of PRP method is higher and the leukocyte contamination is lower than those of BC method are. During storage, the platelet loss is highest in PCs-PRP stored at 4oC in comparison with PCs stored at 22oC. The HSR of PCs-PRP stored at 22oC reached the best results at 1st day and was stable up to 5th day. Conclusion: The PCs-PRP stored at 22oC showed the advantages in platelet yield, the leukocyte contamination and, also, the HSR
