Comparison of the Concentrations of 8-MOP in both Plasma and Suction Blister Fluid after Oral Ingestion.
10.5021/ad.1999.11.4.218
- Author:
Un Cheol YEO
;
Sung Hwan KIM
;
Myoung Min KIM
;
Won Hee JUNG
;
Kyung Eob CHOI
;
Seung Yong JUNG
;
Hyung Geun MIN
- Publication Type:Original Article
- Keywords:
HPLC;
Plasma;
Psoralen;
Suction blister fluid
- MeSH:
Administration, Oral;
Blister*;
Body Weight;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Eating*;
Erythema;
Ficusin;
Humans;
Linear Models;
Methoxsalen*;
Organization and Administration;
Photochemotherapy;
Plasma*;
Skin;
Suction*;
Vitiligo
- From:Annals of Dermatology
1999;11(4):218-224
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The value of plasma concentration of 8-Methoxypsoralen(8-MOP) in the supervision of photochemotherapy has been recognized. However, plasma levels of 8-MOP were not proportionate to the degree of PUVA induced erythema and couldn't alone predict the degree of PUVA induced erythemal reaction. We made a speculation that the degree of PUVA induced erythema might correlate better with skin tissue levels of 8-MOP than plasma levels. Suction blister fluid(SBF) has been known to represent tissue fluid in the skin. So we per-formed a study of comparison of 8-MOP concentrations in both plasma and SBF. OBJECTIVE: Our purpose was to evaluate the correlation of the concentrations of 8-MOP in plasma and SBF 2 hours after oral administration of 0.6 mg/kg of 8-MOP. METHODS: Twenty six patients, aged between 16 and 50 years, undergoing suction blister surgery for vitiligo treatment, participated in this open study. Single oral doses of 0.6 mg/kg of body weight of 8-MOP were taken. Blood samples(5ml) and SBF(2ml) were collected at 2 hours after the drug administration, and 8-MOP concentration in plasma and SBF were quantitated by reverse phase high-performance liquid chromatography (HPLC). RESULTS: 8-MOP concentrations in plasma and SBF ranged from 18 to 545 ng/ml and 8 to 179 ng/ml, respectively. On the analysis of linear regression, a close-relation could not be observed between two SBF levels; measured and predicted values which were calculated from measured plasma and SBF concentrations (r²=0.583, P < 0.001). CONCLUSION: The correlation of plasma and SBF concentrations of 8-MOP is weak. So, SBF levels of psoralen are recommended for the study of PUVA erythemal reactions.
