Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in Saccharomyces cerevisiae.
- Author:
Ngoc Tu NGUYEN
1
;
Yang Hoon KIM
;
Seung Hyuck BANG
;
Ji Hye HONG
;
Soon Dong KWON
;
Jiho MIN
Author Information
- Publication Type:Original Article
- Keywords: Heavy metals; Lysosomal proteomic; Lysosomal response; Mine water; Pharmaceuticals
- MeSH: Environmental Monitoring; Environmental Pollution; Lysosomes; Mass Screening; Metals, Heavy; Saccharomyces cerevisiae*; Sodium; Tetracycline; Water*; Yeasts; Biomarkers
- From:Environmental Health and Toxicology 2014;29(1):e2014019-
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: Lysosome is the cell-organelle which is commonly used as biomonitoring tool in environmental pollution. In this study, the lysosomal proteomic of the yeast Saccharomyces cerevisiae was analyzed for utilization in the detection of toxic substances in mine water samples. METHODS: This work informs the expression of lysosomal proteomic in yeast in response with toxic chemicals, such as sodium meta-arsenite and tetracycline, for screening specific biomarkers. After that, a recombinant yeast contained this biomarker were constructed for toxic detection in pure toxic chemicals and mine water samples. RESULTS: Each chemical had an optimal dose at which the fluorescent protein intensity reached the peak. In the case of water samples, the yeast showed the response with sample 1, 3, 4, and 5; whereas there is no response with sample 2, 6, and 7. CONCLUSIONS: The recombinant yeast showed a high ability of toxic detection in response with several chemicals such as heavy metals and pharmaceuticals. In the case of mine water samples, the response varied depending on the sample content.
