Expression of Basement Membrane Gene in Cultured Skin Fibroblasts from Patients with Diabetes Mellitus.
10.5021/ad.1996.8.1.1
- Author:
Byung Chun KIM
;
Kyu Suk LEE
- Publication Type:Original Article
- Keywords:
Basement membrane gene;
Diabetes Mellitus
- MeSH:
Basement Membrane*;
Blotting, Northern;
Diabetes Mellitus*;
Fibroblasts*;
Fibronectins;
Gene Expression;
Humans;
Laminin;
Procollagen;
RNA;
RNA, Messenger;
Skin*
- From:Annals of Dermatology
1996;8(1):1-5
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Bullous eruption of diabetes(BD) is a rare cutaneous sign of diabetes mellitus(DM). The mechanism for the development of these lesions is unknown, although speculation has ranged from trauma to vascular insufficiency. OBJECTIVE: Our purpose is to evaluate the difference of basement membrane gene expression in cultured skin fibroblasts between patients with diabetes and normal controls. METHODS: Total RNA was extracted from cultured skin fibroblasts of DM and normal, and then Northern blot and slot-blot hybridizations were done. RESULTS: The mRNA levels of a(I) procollagen, a(IV) procollagen, fibronectin, and laminin B1 were not altered significantly in the DM. CONCLUSION: Our results suggest that BD has no relevance to the alteration of basement membrane components. Further studies are needed to clarify the underlying pathogenic mechanism of BD.
