Peptide Mimotopes of Neisseria meningitidis Group B Capsular Polysaccharide.
10.3349/ymj.2004.45.4.755
- Author:
Inho PARK
1
;
In Hong CHOI
;
Se Jong KIM
;
Jeon Soo SHIN
Author Information
1. Department of Microbiology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. jsshin6203@yumc.yonsei.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Neisseria meningitidis;
peptide mimotope;
phage display;
vaccine
- MeSH:
Amino Acid Sequence;
Animals;
Bacterial Vaccines/*immunology;
Cloning, Molecular;
Female;
Meningococcal Infections/*immunology/*prevention & control;
Mice;
Mice, Inbred BALB C;
Molecular Sequence Data;
Neisseria meningitidis, Serogroup B/genetics/*immunology;
Polysaccharides, Bacterial/genetics/*immunology;
Research Support, Non-U.S. Gov't
- From:Yonsei Medical Journal
2004;45(4):755-758
- CountryRepublic of Korea
- Language:English
-
Abstract:
The antigenic similarity between Neisseria meningitidis group B (NMGB) capsular polysaccharide (PS) and human polysialic acid (PSA) has hampered the development of a NMGB PS-based vaccine. But the possibility of a safe vaccine based on NMGB PS has been demonstrated by the existence of the NMGB PS-associated nonautoreactive epitope, which is distinct from those present on human PSA. To obtain peptide mimotopes of NMGB PS, we used HmenB3, a protective and nonautoreactive monoclonal antibody, to screen a phage library with 12 amino acids. We obtained 23 phage clones that bound to HmenB3 but not in the presence of E. coli K1 PS [which is alpha (2-8) -linked PSA like NMGB PS]. The clones contained 3 mimotopes and differed from previously described NMGB PS mimotopes. Immunization with a synthetic peptide of one mimotope elicited anti-NMGB antibodies in BALB/c mice. These mimotopes may be useful in the development of group B meningococcal vaccines.
