Utility of In-House PCR for HLA-B27 Typing: Comparison of Concordance Rate between PCR Kit and In-House PCR.
10.3343/kjlm.2008.28.3.239
- Author:
Sun Young CHO
1
;
Kwang Gil LEE
;
Su Yon PARK
;
Hee Joo LEE
Author Information
1. Department of Laboratory Medicine, Kyung-Hee Medical Center, School of Medicine, Kyoung-Hee University, Seoul, Korea. leehejo@khmc.or.kr
- Publication Type:Original Article ; Comparative Study ; English Abstract ; Evaluation Studies
- Keywords:
HLA-B27;
In-house PCR;
DNA typing
- MeSH:
Adult;
Female;
HLA-B27 Antigen/blood/*genetics;
Histocompatibility Testing/*methods;
Humans;
Male;
Polymerase Chain Reaction/*methods;
Reagent Kits, Diagnostic;
Sensitivity and Specificity
- From:The Korean Journal of Laboratory Medicine
2008;28(3):239-243
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Commercial kits of PCR method are widely used in HLA-B27 typing; however, their cost is relatively high. In this study, we evaluated the utility of an in-house PCR method by comparing it with that of a commercial kit. METHODS: HLA-B27 typing was done in 188 patients by using two PCR methods, Absolute(TM) HLAB27 PCR kit (Biosewoom, Korea) and an in-house PCR method. The primers used in the in-house method were prepared by Bioneer (Korea). Both PCR tests were done by Gene Amp PCR System 9600 (Perkin-Elmer Centus Corp., USA). RESULTS: The commercial kit and in-house PCR showed 100% concordance rate with each other in HLA-B27 typing. Of 188 patients tested 72 (38.3%) were positive and 116 (61.7%) were negative by the both tests. Of 62 patients with ankylosing spondylitis, 50 were positive (80.7%). CONCLUSIONS: The in-house PCR is a reliable and cost-effective method and can replace or supplement commercial kits for HLA-B27 typing.
