Cytokine expression pattern in milk somatic cells of subclinical mastitis-affected cattle analyzed by real time PCR.
- Author:
Vaibhav D BHATT
1
;
Prasad S KHADE
;
Sagar B TARATE
;
Ajai K TRIPATHI
;
Dev S NAURIYAL
;
Dharamshi N RANK
;
Anju P KUNJADIA
;
Chaitanya G JOSHI
Author Information
1. Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, Anand 388120, Gujarat, India. bhatt_vbhv@yahoo.co.in
- Publication Type:Original Article
- Keywords:
cytokines;
interleukins;
interferon;
quantitative real time PCR
- MeSH:
Animals;
Bacteria;
Bacterial Infections;
Bacterial Load;
Cattle;
Cell Count;
Cytokines;
Female;
Granulocytes;
Interferon-gamma;
Interferons;
Interleukin-12;
Interleukin-8;
Interleukins;
Mammary Glands, Human;
Mastitis;
Mastitis, Bovine;
Milk;
Real-Time Polymerase Chain Reaction;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Veterinary Research
2012;52(4):231-238
- CountryRepublic of Korea
- Language:English
-
Abstract:
The expression profiles of inflammatory cytokines viz. interleukins (IL)-6, IL-8, IL-12, granulocyte macrophage-colony stimulating factor, interferon-gamma and tumor necrosis factor-alpha in response to subclinical mastitis in indigenous cattle breed Kankrej (n = 6), Gir (Bos indicus) (n = 12) and crossbred (Bos taurus x Bos indicus) (n = 7) were investigated using quantitative real time PCR. Significant correlation (p < 0.05) was observed between total bacterial load and somatic cell count (SCC) in all three breeds of cattle. All the cytokines were observed to be up-regulated compared to cows with healthy quarters, however, level of their expression varied among three breeds of cattle. In Kankrej most cytokines were found to be transcribed to higher levels than in other two breeds; the milk had higher load of bacteria but not so high SCC, implying that Kankrej has a higher inherent resistance against mastitis. The results of present study indicated that mammary glands of crossbred cattle are more sensitive to bacterial infection than indigenous breed of cattle as they elicit immune response at lower bacterial load and result into higher SCC. Research on identification of factors responsible for differentially expressed cytokines profiles and use of cytokines as immunomodulatory tools can pave way for formulating control strategies against bovine mastitis.
