Analysis of in vitro apoptosis induced by virulent Korean isolate of classical swine fever virus in peripheral blood B cell line.
- Author:
Seon Mi KIM
1
;
Seong In LIM
;
Jae Young SONG
;
Bang Hun HYUN
Author Information
1. Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-757, Korea. hyunbh@korea.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
apoptosis;
classical swine fever virus;
lymphopenia;
L35
- MeSH:
Animals;
Apoptosis;
bcl-X Protein;
Caspase 3;
Cell Line;
Classical Swine Fever;
Classical swine fever virus;
Humans;
Korea;
Leukopenia;
Lymphopenia;
Phenylurea Compounds;
Sprains and Strains;
Swine
- From:Korean Journal of Veterinary Research
2012;52(4):259-262
- CountryRepublic of Korea
- Language:English
-
Abstract:
Classical swine fever (CSF) is a highly contagious disease among swine that has an important economic impact on worldwide. One clinical symptom of CSF is leukopenia, in particular lymphopenia, which is a characteristic event that occurs early in the course of CSF. Though lymphopenia associated with apoptosis, the pathogenic mechanism underlying the lymphopenia has not been well studied. To understand these mechanisms, we investigated the response of porcine B cell lines to infection with SW03, virulent strain isolated from swine tissue in Korea. This study demonstrated that SW03-infected L35 cell were induced apoptosis through the detection of activated caspase-3. In addition, SW03 infection leaded to alterations in pro-apoptotic, Bax, and anti-apoptotic, Bcl-xL proteins of Bcl-2 family. Our results would suggest that SW03-infected L35 cells induced apoptosis via intrinsic mitochondrial pathway.
