The Anti-Inflammatory Effect of Pegmatite by in Vivo and in Vitro Study.
- Author:
Min Hyuk LEE
1
;
Seok Kwun KIM
;
Yong Seok KWON
;
Jang Ho LEE
;
Keun Cheol LEE
Author Information
1. Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University, Busan, Korea. pokdungi@dau.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Pegmatite;
Anti-inflammatory activity;
Ear edema;
Vascular permeability;
NO;
LOX
- MeSH:
Acetic Acid;
Animals;
Arachidonic Acid;
Capillary Permeability;
Ear;
Edema;
Interleukin-6;
Macrophages;
Mice;
Models, Animal;
Nitric Oxide;
Tumor Necrosis Factor-alpha
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2010;37(1):12-21
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This work aimed to elucidate the anti-inflammatory effect of pegmatite in vitro and in vivo. METHODS: Author evaluated the suppressive effects of pegmatite on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production, TNF-alpha and IL-6 release in the RAW 264.7 murine macrophages. RESULTS: Treatment of RAW 264.7 cells with pegmatite significantly reduced LPS-stimulated NO production and inflammatory cytokine such as TNF-alpha and IL-6 secretion in a concentration-dependent manner. Also pegmatite showed topical anti-inflammatory activity in the arachidonic acid (AA)-induced ear edema and acetic acid-induced increase in capillary permeability assessment in mice. It was also found that pegmatite (10 mg per ear in DW) inhibited arachidonic acid induced edema at 24 h more profoundly than 1 h by topical application. Furthermore, the vascular permeability increase induced by acetic acid was significantly reduced in mice that received pegmatite in 50 mg per mouse. CONCLUSION: Therefore the results of the present study suggest that pegmatite is a potent inhibitor of the LPSinduced NO and inflammatory cytokine in RAW 264.7 macrophages and showed anti-inflammatory activities in vivo animal model.
